Saturday, 10 October 2015

08:30 - 10:45     Session 2A: Molecular Virology – Entry                                    Salle Erasme
                          Session chairs: François-Loïc COSSET & Matt EVANS

08:30 – 09:00 Plenary lecture: Didier TRONO

tronoTransposable elements, polydactyl proteins and the establishment of human-specific transcriptional network
Didier TRONO, MD, Professor of Genetics and Virology, Laboratory of Virology and Genetics, Ecole Polytechnique Fédérale de Lausanne (EPFL), School of Life Sciences, Global Health Institute, Lausanne, Switzerland

Prof. Trono completed clinical training in pathology, internal medicine and infectious diseases in Geneva and at Massachusetts General Hospital before performing post-doctoral work with David Baltimore at the Whitehead Institute. In 1990, he moved to the Salk Institute to launch a center for AIDS research. From 1997 to 2004, he was at the University of Geneva as professor and then head of the Department of Genetics and Microbiology. He then joined the EPFL as dean of its newly launched School of Life Sciences, a position he held for eight years. He now leads the Health 2030 Initiative, a joint venture of the EPFL, the Universities of Geneva and Lausanne and their affiliated hospitals to promote the advent of personalized health in Switzerland.
Didier Trono’s research has long gravitated around interactions between viruses and their hosts and the development of tools for gene therapy. This led him to epigenetics, and to explore the impact of transposable elements and their controllers on transcriptional networks governing human development and physiology.

 

09:00 - 09:15     Primer HCV Entry
                          Thomas PIETSCHMANN, Twincore, Hannover, Germany

09:15 - 10:45 Session 2A oral abstracts

09:15 - 09:30 High-throughput mutagenesis analysis of recombinant hepatitis C virus envelope glycoprotein complex E1E2 O2.01
  Kelli JACKSON, Andrew ETTENGER, Johnathan GUEST, Jennifer M PFAFF, Trevor BARNES, Erick GIANG, Edgar DAVIDSON, Benjamin J DORANZ, Mansun LAW  
09:30 - 09:45 A protein coevolution method uncovers critical features of the fusion mechanism during hepatitis C virus entry into host cells O2.02
  Florian DOUAM, Floriane FUSIL, Margot ENGUEHARD, Linda DIB, Loic SCHWALLER, Jimmy MANCIP, Laurent MAILLY, Els VERHOYEN, Thomas F BAUMERT, Alessandra CARBONE, François-Loïc COSSET, Dimitri LAVILLETTE  
09:45 - 10:00 Hypervariable region 1 shielding of hepatitis C virus occurs in vivo and is a major contributor to genotypic differences in neutralization sensitivity
O2.03
  Jannick PRENTOE, Rodrigo MOCTEZUMA, Lieven VERHOYE, Caroline BUYSSCHAERT, Ali FARHOUDI, Richard WANG, Harvey ALTER, Erick GIANG, Zhenyong KECK, Steven KH Foung, Mansun LAW, Philip MEULEMAN, Jens BUKH  
10:00 - 10:15 Serum-response factor binding protein 1 is a HCV host co-factor required for cell entry O2.04
  Gisa GEROLD, Felix MEISSNER, Janina BRUENING, Kathrin WELSCH, Paula M PERIN, Thomas F BAUMERT, Florian W VONDRAN, Lars KADERALI, Joseph MARCOTRIGIANO, Abdul G KHAN, Matthias MANN, Charles M RICE, Thomas PIETSCHMANN  
10:15 - 10:30 Roles of lipoprotein receptors in the entry of hepatitis C virus O2.05
  Takasuke FUKUHARA, Yamamoto SATOMI, Ono CHIKAKO, Mori HIROYUKI, Puig FRANCESC, Okamoto TORU, Yoshiharu Matsuura  
10:30 - 10:45 Potent, specific inhibitors of hepatitis C virus p7 define a functional requirement for channel activity during virus entry O2.06
  Joseph SHAW, Rajendra GOSEIN, Toshana FOSTER, Jayakanth KANKANALA, Richard FOSTER, Stephen GRIFFIN  

 

10:45 - 11:15     Refreshment Break                                                                    Forum

11:15 - 12:45     Session 2B: Molecular Virology – Assembly
                          Session chairs: Philippe Roingeard & Catherine Schuster

11:15 - 11:30     Video of HCV life cycle
                          Darius MORADPOUR, University of Lausanne, Lausanne, Switzerland & François PENIN, University of Lyon, Lyon, France

11:30 - 12:45 Session 2B oral abstracts

11:30 - 11:45 Domain III of NS5A contains two regions regulating different steps of HCV assembly O2.08
  Margarita ZAYAS, Gang LONG, Vanesa MADAN, Ralf BARTENSCHLAGER  
11:45 - 12:00 Novel role for Nup98 in HCV assembly
O2.09
  Marion LUSSIGNOL, Gema VIZCAY, Roland FLECK, Maria Teresa CATANESE  
12:00 - 12:15 Annexin A3 is recruited to lipid droplets in HCV-infected cells and regulates HCV particle production
O2.10
  Kathrin ROESCH, Anja SCHOEBEL, Cordula GRUETTNER, Marcel KWIATKOWSKI, Hartmut SCHLUETER, Eva HERKER  
12:15 - 12:30 HCV infection generates a novel Rab7 binding protein that promotes outward trafficking and virion secretion O2.11
  Ann L WOZNIAK, Steven A WEINMAN  
12:30 - 12:45 Functional and biochemical characterization of HCV particles produced in a humanized liver mouse model O2.12
  Sara CALATTINI, Floriane FUSIL, Jimmy MANCIP, Viet Loan DAO THI, Christelle GRANIER, Nicolas GADOT, Jean-Yves SCOAZEC, Dimitri LAVILLETTE, Marlène DREUX, François-Loïc COSSET  

 

13:00 - 14:30     Lunch                                                                                         Galerie de Marbre

14:30 - 16:30     Session 2C: Molecular Virology – Translation/Replication
                          Session chairs: Volker LOHMANN & Brett LINDENBACH

14:30 – 15:00 Keynote lecture: Ralf BARTENSCHLAGER

Ralf BartenschlagerOn the complexities of interactions between the hepatitis C virus and its host cell: a brief comparison with Dengue virus
Ralf Bartenschlager, PhD, Professor of Molecular Virology, Head of the Department of Infectious Diseases, Molecular Virology, Heidelberg University, Head of the Division “Virus-associated carcinogenesis”, German Cancer Research Center, Heidelberg, Germany

Prof. Bartenschlager studied biology at Heidelberg University, Germany. After his diploma work (1987) and PhD thesis (1990) at the Center of Molecular Biology (ZMBH), Heidelberg, Germany he joined as a postdoctoral fellow Hoffmann-La Roche, Basel, Switzerland, where he started to work on hepatitis C virus (HCV). Back to Germany he joined the University of Mainz in 1993 and worked there as an independent research group leader. In May 2002 the Heidelberg Faculty of Medicine appointed him as a full professor, endowed by the Chica and Heinz Schaller Foundation where since then he is head of the Department of Infectious Diseases, Molecular Virology. As of 2014 he is also heading the research unit “Virus-associated Carcinogenesis” at the German Cancer Research Center (DKFZ) Heidelberg. His research unit, which is part of the Heidelberg University Hospital concentrates on the following research areas: (1) analyzing and comparing the replication cycles of an acute lytic Dengue Virus (DENV)) and a chronic persistent virus (hepatitis C virus (HCV)) to understand how these viruses interfere with the immune response, especially the innate immune response and how they exploit the host cell machineries to establish their membranous replication factories; (2) understanding the spatio-temporal coupling of the different steps of the replication cycles of these viruses; (3) development of dynamic models for cellular networks responsible for HCV and DENV replication, spread and counteraction of innate immune response; (4) studying the long-term consequences of persistent viral infection with respect to host cell damage and, in case of HCV, liver cancer development; (5) studying the host-pathogen interaction through RNAi-based high-throughput screening in combination with bioinformatic and systems biology approaches to reveal relevant cellular networks.

 

15:00 - 16:30 Session 2C oral abstracts

15:00 - 15:15 Ultrastructural alterations induced by HCV replication revealed by cryo soft X-ray tomography O2.13
  Ana PEREZ-BERNA, Maria Jose RODRIGUEZ, Francisco Javier CHICHóN, Martina FRIESLAND, Andrea SORRENTINO, Jose Lopez CARRASCOSA, Eva PEREIRO, Pablo GASTAMINZA  
15:15 - 15:30 New insights into the building blocks of the hepatitis C virus replication organelles
O2.14
  Ines ROMERO-BREY, Carola BERGER, Stephanie KALLIS, Androniki KOLOVOU, David PAUL, Volker LOHMANN, Ralf BARTENSCHLAGER  
15:30 - 15:45 Phosphorylation of NS5A Ser-235 is essential to HCV RNA replication and normal replication compartment formation
O2.15
  Nicholas EYRE, Rachel HAMPTON-SMITH, Amanda ALOIA, Kaylene SIMPSON, James EDDES, Peter HOFFMAN, Michael BEARD  
15:45 - 16:00 Expression, purification, characterization and membrane reconstitution of nonstructural protein 2 from hepatitis C virus O2.16
  Marie-Laure FOGERON, David PAUL, Susanne PENZEL, Vlastimil JIRASKO, Roland MONTSERRET, Denis LACABANNE, Aurelie BADILLO, Celia BOUKADIDA, Annette MARTIN, Beat MEIER, Ralf BARTENSCHLAGER, François PENIN, Anja BOCKMANN  
16:00 - 16:15 Experimentally distinguishable compartments in the membranous web supporting different replicative activities O2.17
  Rafael GOMES, Olaf ISKEN, Norbert TAUTZ, John McLAUCHLAN, Christopher McCORMICK  
16:15 - 16:30 Proteomics of HCV virions O2.17
  Maria Teresa CATANESE, Martina KOPP, Kelly MOLLOY, Marcus DORNER, Kierstin SCHMIDT, Brian T CHAIT, Charles M Rice  

 

16:30 - 18:30     Poster Session I                                                                         Forum
                                
Wine & cheese